KLN 205細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： CRL-1453?
相關(guān)**： 鱗狀細(xì)胞癌
生長狀態(tài)： 貼壁生長
品系： DBA/2
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 肺
KLN 205細(xì)胞Designations: KLN 205
Depositors: T Kaneko
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Organ: lung
Strain: DBA/2
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. KLN 205細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: KLN 205 cells form metastatic lesions in lungs after inoculation into mice.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: KLN 205細(xì)胞A subcultivation ratio of 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: lquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1074: Kaneko T, LePage GA. Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res. 38: 2084-2090, 1978. KLN 205細(xì)胞PubMed: 418873
