IgG-11H4細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

數(shù)量： 大量

ATCC Number： CRL-1936?

細胞形態(tài)： **樣

**類型： IgG1

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

IgG-11H4細胞Designations: IgG-11H4

Depositors: YK Ho, JL Goldstein

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: IgG-11H4細胞immunoglobulin; monoclonal antibody; against the carboxy terminus of lipoprotein receptor related protein (LRP)

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Comments: Animals were immunized with a synthetic peptide (corresponding to the 13 carboxy terminal peptides of human LRP) coupled to KLH.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with the carboxy terminus of LRP from various species.

Propagation: IgG-11H4細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: 1 to 2 times per week

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: IgG-11H4細胞complete growth medium, 95%; DMSO, 5%

References: 23314: Kowal RC, et al. Low density lipoprotein receptor-related protein mediates uptake of cholesteryl esters derived from apoprotein E-enriched lipoproteins. Proc. Natl. Acad. Sci. USA 86: 5810-5814, 1989. PubMed: 2762297

23553: Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029