HeLa細(xì)胞

器官來源： 宮頸

生長狀態(tài)： 貼壁生長

年限： 31 years *****

運輸方式： 凍存運輸

ATCC Number： CCL-2?

相關(guān)**： 腺癌

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

細(xì)胞類型： 上皮細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 人

HeLa細(xì)胞Designations: HeLa

Depositors: WF Scherer

Biosafety Level: 2 [Cells contain human papilloma virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cervix

Disease: adenocarcinoma

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:HeLa細(xì)胞 transfection host

screening for Escherichia coli strains with invasive potential

Virus Susceptibility: Human adenovirus 3

Encephalomyocarditis virus

Human poliovirus 1

Human poliovirus 2

Human poliovirus 3

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Cytogenetic Analysis: Modal number = 82; range = 70 to 164.

There is a small telocentric chromosome in 98% of the cells. 100% aneuploidy in 1385 cells examined. Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. HeLa細(xì)胞M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 is a combination of short arm of chromosome 3 and long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 consists of the long arm of chromosome 11 and an arm of chromosome 19.

Isoenzymes: G6PD, A

Age: 31 years *****

Gender: female

Ethnicity: Black

HeLa Markers: Y

Comments: The cells are positive for keratin by immunoperoxidase staining.

HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.

P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

HeLa細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

also available as Certified Reference Material, ATCC CRM-CCL-2

derivative:ATCC CCL-2.1

derivative:ATCC CCL-2.2

derivative:ATCC CCL-2.3

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