FRTL細(xì)胞

ATCC Number： CRL-1468?

相關(guān)**： 正常

器官來源： 甲狀腺

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

品系： Fischer

年限： 6 weeks

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

FRTL細(xì)胞細(xì)胞形態(tài)： 上皮樣

Designations: FRTL

Depositors: HG Coon

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, patchy

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Strain: Fischer

Organ: thyroid

Disease: normal

Cellular Products: thyroglobulin

Permits/Forms: FRTL細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Cytogenetic Analysis: diploid

Age: 6 weeks

Comments: The cells accumulate iodide intracellularly.

Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with:10 mU/ml TSH, 0.01 mg/ml insulin10 nM hydrocortisone 0.005 mg/ml transferrin10 ng/ml somatostatin10 ng/ml glycyl-L-histidyl-L-lysine acetate0.5 % bovine calf serum(All are obtainable from Sigma, Collaborative Research and/or Calbiochem.)

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: FRTL細(xì)胞Protocol: There is an absolute requirement for 0.1 to 0.5% calf serum in addition to insulin, hydrocortisone, thyrotropin, transferrin, somatostatin and glycyl-1-histidyl-lysine acetate.

The cells tend to grow one above another, forming three dimensional structures rather than expanding into a monolayer.

Subculture before ?domes? form, flasks never become confluent.

Remove and discard culture medium.

Add 2.0 to 3.0 ml of CTC solution ( Collagenase, 20 U/ml; Trypsin, 0.075%; dialyzed heat-inactivated Chicken serum, 2%) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-collagenase solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37?C.

Subcultivation Ratio: FRTL細(xì)胞A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2030

References: 1066: Ambesi-Impiombato FS, et al. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: 3455-3459, 1980. PubMed: 6106191

23211: Bidey SP, et al. Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin. J. Endocrinol. 101: 269-276, 1984. PubMed: 6327870