MMQ細胞
細胞形態(tài)： 上皮樣
數量： 大量
器官來源： 垂體
ATCC Number： CRL-10609?
相關**： 腫瘤
年限： 5 days
是否是腫瘤細胞： 0
物種來源： 褐鼠
運輸方式： 凍存運輸
生長狀態(tài)： 懸浮生長
MMQ細胞Designations: MMQ
Depositors: University of Virginia Patent Foundation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus
Morphology: epithelial
Source: Organ: pituitary
Disease: tumor
Cellular Products: prolactin
Permits/Forms: MMQ細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: dopamine
Tumorigenic: No
Age: 5 days
Comments: The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 2.5%
horse serum to a final concentration of 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: MMQ細胞37.0°C
DGrowth Conditions: The line grows as clumps of cells in suspension.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 to 48 hrs
Related Products: MMQ細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X
References: 22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996
