Pro-5細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

ATCC Number： CRL-1781?

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 單層（少量懸?。?

器官來源： 卵巢

細(xì)胞形態(tài)： 上皮樣

Designations: Pro-5

Depositors: P Stanley

Pro-5細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer or suspension

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Pro-5細(xì)胞The parental Pro-5 line was deposited by Dr. P. Stanley. The cells are not available commercially and it is requested that: 1) In all papers using the cell line a direct reference will be made to Stanley et al. Cell 6:121-128, 1975. 2) Any proposed commercial use of the cells must be negotiated with Dr. P. Stanley, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. 3) No distribution of line derived from the Pro-5 line (including those derived by hybridization and/or transfection) be made to third parties without Dr. Stanley's permission

Gender: female

Comments: This line is a derivative of the original CHO cell line and is the parental line for several glycosylation mutants (see ATCC CRL-1735, 1736 and 1737).

The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).

The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.

Propagation: Pro-5細(xì)胞ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature:Pro-5細(xì)胞 liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 1201: Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798