NN-4細(xì)胞

ATCC Number： HB-8473?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

NN-4細(xì)胞Designations: NN-4

Depositors: Lawrence Livermore National Lab

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: NN-4細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human glycophorin A type N

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Comments: Animals were immunized with type O, Rh- human erythrocytes and purified human glycophorin A.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: NN-4細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: NN-4細(xì)胞1098: Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283