5E9C11細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HB-21?

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

Designations: 5E9C11

Depositors: A Fauci, BF Haynes

Isotype: IgG1; kappa light chain

Biosafety Level: 1

5E9C11細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte); also reacts with human transferrin receptor

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Tumorigenic: Yes

Comments: 5E9C11細(xì)胞Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).

Spleen cells were fused with P3X63Ag8 myeloma cells.

This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.

The antibody recognizes a 90000 dalton cell surface antigen (the same as that recognized by OKT 9, ATCC CRL-8021).

The antibody precipitates human transferrin receptor.

It does not fix complement.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:5E9C11細(xì)胞 Medium Renewal: Every 2 to 3 days

This line has carried by passage as ascites in pristane primed (0.5 ml, 7 days prior to injection of cells) mice.

The mice are injected with 0.3 to 0.5 ml of cells, and the ascites is collected 7 to 10 days later.

Recent information from the donor indicates that the cells are readily grown in culture using the above medium.

Cultures can be maintained by addition or replacement of fresh medium.

Start cultures at 5 X 10 exp4 cells/ml and do not allow the cells to exceed 8 X 10 exp5 cells/ml.

References: 915: Haynes BF, et al. 5E9C11細(xì)胞Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J. Immunol. 127: 347-351, 1981. PubMed: 6787129