CSMalphabeta6C細(xì)胞

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 乳腺

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

品系： RIII

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 成纖維樣

ATCC Number： CRL-8400?

相關(guān)**： 腫瘤

CSMalphabeta6C細(xì)胞Designations: CSMalphabeta6C [CSMab6C]

Depositors: Genzyme Corp.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Strain: RIII

Organ: mammary gland

Disease: carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CSMalphabeta6C細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: produces chorionic gonadotropin, beta polypeptide

produces glycoprotein hormones, alpha polypeptide chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha

Reverse Transcript: N

Gender: female

HeLa Markers: N

Comments: This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.

The cells produce very high levels of alpha and beta hCG.

The line contains the plasmid pRF 398 alpha t2.

The construct contains the entire sequence of beta hCG plus 30 bp 5' untranslated and 60 bp 3' untranslated under the control of the mouse metallothionein promoter. [12607]

The construct contains the entire sequence of alpha hCG plus 10 bp 5' untranslated and 220 bp 3' untranslated under the control of the SV40 early promoter. CSMalphabeta6C細(xì)胞[12607]

An EcoRI/BamHI fragment of palphat2 (containing alpha hCG and SV40 sequences) was inserted in the BamHI site of pRF398 (ATCC CRL 8401) (containing beta hCG, MT-1 promoter, and BPV sequences) to create pRF398alphat2. [12607]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: CSMalphabeta6C細(xì)胞parental cell line:ATCC CRL-1616

References: 12607: Reddy VB, et al. Heteropolymeric protein. US Patent 4,840,896 dated Jun 20 1989