LBRM-33-1A5細(xì)胞

品系： B10.BR

ATCC Number： CRL-8079?

數(shù)量： 大量

相關(guān)**： **瘤

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

器官來源： 脾

細(xì)胞形態(tài)： **樣

Designations: LBRM-33-1A5

LBRM-33-1A5細(xì)胞Depositors: S Gillis

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Strain: B10.BR

Organ: spleen

LBRM-33-1A5細(xì)胞Disease: lymphoma

Cellular Products: interleukin-2 (interleukin 2, IL-2)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: interleukin 1 (IL-1)

Comments: The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.

Propagation: LBRM-33-1A5細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 0.0025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Maintain at approximately 8 X 10 exp5 viable cells/ml.

References: 3528: Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. LBRM-33-1A5細(xì)胞US Patent 4,404,280 dated Sep 13 1983