IM-9細胞

ATCC Number： CCL-159?

器官來源： 外周血

數量： 大量

生長狀態(tài)： 懸浮生長

細胞類型： B**細胞

是否是腫瘤細胞： 0

物種來源： 人

運輸方式： 凍存運輸

細胞形態(tài)： **樣

IM-9細胞**類型： IgG kappa , IgG; kappa light chain [26021]

Designations: IM-9

Depositors: DN Buell

Isotype: IgG kappa , IgG; kappa light chain [26021]

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: IM-9細胞lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1967

Receptors: growth hormone

insulin

calcitonin

growth hormone; insulin; calcitonin

Antigen Expression: CD11a +; CD19 +; CD20 +; CD38 -; CD49e +

DNA Profile (STR): IM-9細胞Amelogenin: X

CSF1PO: 10,11

D13S317: 9,11

D16S539: 9,13

D5S818: 13

D7S820: 11,12

THO1: 6,9.3

TPOX: 11

vWA: 14,17

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes: ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 2

PGM1, 1-2

PGM3, 0

Gender: female

Ethnicity: Caucasian

Comments: Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]

The cells are EBNA positive.

Propagation: IM-9細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1-2 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Do not allow the cell density to exceed 2 X 10 exp6 cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1031: Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016

1035: Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817

1225: Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222

1226: Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334

26021: van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762