NZP-12細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 肺

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CRL-1921?

是否是腫瘤細(xì)胞： 0

物種來源： 其他

NZP-12細(xì)胞Designations: NZP-12

Depositors: L Munson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Crossarchus obscurus

Morphology: fibroblast

NZP-12細(xì)胞

Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The cells can be propagated for at least 13 population doublings beyond frozen reference seed stock aspirate and dispense into new flasks.

Propagation: NZP-12細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 45%; Ham's F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). NZP-12細(xì)胞Add fresh medium.