M2-10B4細(xì)胞

生長狀態(tài)： 貼壁生長

組織來源： stroma

細(xì)胞形態(tài)： 成纖維樣

器官來源： 骨髓

ATCC Number： CRL-1972?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

Designations: M2-10B4

M2-10B4細(xì)胞Depositors: CJ Eaves

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: bone marrow

Tissue: stroma

Cellular Products: laminin; collagen IV

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. M2-10B4細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a (C57BL/6J X C3H/HeJ)F1 mouse.

The cells express laminin and collagen IV, but do not express collagen I or Factor VIII.

The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. M2-10B4細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22620: Sutherland HJ, et al. Differential regulation of primitive human hematopoietic cells in long- term cultures maintained on genetically engineered murine stromal cells. Blood 78: 666-672, 1991. PubMed: 1713512

22769: Lemoine FM, et al. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp. Hematol. 16: 718-726, 1988. PubMed: 3261251

23070: Lemoine FM, et al.M2-10B4細(xì)胞 Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Cancer Res. 48: 6438-6443, 1988. PubMed: 3263188

23353: Lemoine FM, et al. Transformation-associated alterations in interactions between pre-B cells and fibronectin. Blood 76: 2311-2320, 1990. PubMed: 2147863