DT95細(xì)胞

細(xì)胞形態(tài)： **樣

ATCC Number： CRL-2112?

相關(guān)**： **瘤

是否是腫瘤細(xì)胞： 0

物種來源： 其他

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

組織來源： bursa

Designations: DT95

DT95細(xì)胞Depositors: EH Humphries

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Gallus gallus

Morphology: lymphoblast

Source: Tissue: bursa

Disease: lymphoma

Cellular Products: immunoglobulin; IgM (surface and secreted)

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Tumorigenic: DT95細(xì)胞Yes

Oncogene: c-myc +

Comments: DT95 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken.

The original lymphoma was induced by viral infection of a 1 day old chicken with Rous associated virus 1 (RAV-1).

Cell suspensions prepared from tumors that developed within the bursa of Fabricus were transferred intravenously into young syngeneic recipient chickens.

After one transfer in vivo, the DT95 cell line was established.

The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA.

In vitro, both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class I, MHC class II, and interleukin-2 receptor (IL-2R) antigens on the DT95 cell line.

The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel.

The cell line exhibits a more differentiated plasmacytoid phenotype than DT40 (see ATCC CRL-2111).

Infectivity assays revealed that DT95 does not release infectious RAV-1.

Propagation: DT95細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 0.05 mM 2-mercaptoethanol, 75%; tryptose phosphate broth, 10%; fetal bovine serum, 10%; chicken serum, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml.

Interval: Maintain between 2 X 10(5) and 2 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

References: 22571: Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742

22724: Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. DT95細(xì)胞PubMed: 1490388

22994: Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040