RSC96細(xì)胞

ATCC Number： CRL-2765?

細(xì)胞形態(tài)： 神經(jīng)元

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 褐鼠

運(yùn)輸方式： 凍存運(yùn)輸

Designations: RSC96

Depositors: PI Patel

RSC96細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus

Morphology: neuronal

Source: Cell Type: neuronal Schwann cell; spontanous immortalization

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Comments: Oncogene:

Eerb-B2, positive RSC96細(xì)胞[PubMed: 9766530]Erb-B3, negative [PubMed: 9766530]Products:

cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive[PubMed: 9766530]laminin; positive [PubMed: 9766530]Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843] Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]peripheral myelin protein 22 (Pmp22); mRNA, positive; protein, negative [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors expressed:

Nerve growth factor receptor (Ngfr); Protein and mRNA, negative [PubMed: 12210843]platelet derived growth factor receptor, alpha; negative [PubMed: 9766530]platelet derived growth factor receptor, beta; negative [PubMed: 9766530]

Comments:

The RSC96 cell line is a spontaneously transformed rat Schwann cell line derived from long-term culture of rat primary Schwann cells [PubMed: 9766530].A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Propagation: RSC96細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium:RSC96細(xì)胞 Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843

71856: Badache A, De Vries GH. Neurofibrosarcoma-derived Schwann cells overexpress platelet-derived growth factor (PDGF) receptors and are induced to proliferate by PDGF BB. J. Cell. Physiol. 177: 334-342, 1998. PubMed: 9766530