PU5-1.8 (PU5-1R)細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長，少量貼壁
運(yùn)輸方式： 凍存運(yùn)輸
品系： BALB/c
ATCC Number： TIB-61?
數(shù)量： 大量
相關(guān)**： 其他**
Designations: PU5-1.8 (PU5-1R)
Depositors: P Ralph
PU5-1.8 (PU5-1R)細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension (some adherent cells)
Organism: Mus musculus
Morphology:
Source: Disease: lymphoid tumor
Strain: BALB/c
Cellular Products: lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. PU5-1.8 (PU5-1R)細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: complement (C3)
Comments: PU5-1.8 cells phagocytose latex beads and zymosan.
The cells are capable of antibody dependent lysis of both sheep erythrocytes and tumor cells.
The line is sensitive to growth inhibition by LPS and PPD.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: PU5-1.8 (PU5-1R)細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Adherent cells can be recovered by scraping.
References: 1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890
1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PU5-1.8 (PU5-1R)細(xì)胞PubMed: 318922
1137: Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429
