RPMI 1788細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

器官來源： 外周血

年限： 33 years

細(xì)胞形態(tài)： **樣

**類型： IgM; lambda light chain

ATCC Number： CCL-156?

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

RPMI 1788細(xì)胞物種來源： 人

Designations: RPMI 1788

Depositors: GE Moore

Isotype: IgM; lambda light chain

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)

Permits/Forms: RPMI 1788細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: HLA A2, Aw33, B7, B14

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 10,12

THO1: 6,9.3

TPOX: 8,9

vWA: 18,19

Cytogenetic Analysis: human male; hypodiploid; stable

Isoenzymes: G6PD, B

Age: 33 years

Gender: male

Ethnicity: Caucasian

Comments: RPMI 1788細(xì)胞The cells are EBNA positive.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium.Cultures are established at 3 X 10 exp5 viable cells/ml.

References: 22796: Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523

22918: Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807

23213: Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. RPMI 1788細(xì)胞PubMed: 5353241