NR8383 [AgC11x3A, NR8383.1]細胞

運輸方式： 凍存運輸

器官來源： 肺

數(shù)量： 大量

品系： Sprague-Dawley

細胞形態(tài)： 單核細胞/巨噬細胞

細胞類型： 其他細胞類型

生長狀態(tài)： 混合型生長

ATCC Number： CRL-2192?

相關**： 正常

是否是腫瘤細胞： 0

物種來源： 大鼠

NR8383 [AgC11x3A, NR8383.1]細胞Designations: NR8383 [AgC11x3A, NR8383.1]

Depositors: RJ Helmke

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: macrophage

Source: Organ: lung

Strain: Sprague-Dawley

Disease: normal

Cell Type: macrophage (alveolar);

Cellular Products: transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6)

Permits/Forms: NR8383 [AgC11x3A, NR8383.1]細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: August 3, 1983

Applications: NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).

Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.

The cells exhibit characteristics of macrophage cells:

The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.

The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities.

These cells are sensitive to endotoxin.

Receptors: Fc

Comments: NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage.

NR8383 [AgC11x3A, NR8383.1]細胞The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.

Subsequently the requirement for exogenous growth factors was lost.

NR8383 cells were cloned and subcloned from single cells by limiting dilution, and then subcloned from soft agar three times.

The cells exhibit characteristics of macrophage cells:

Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.

The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.

NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).

Stimulation with bleomycin also increases TGF beta mRNA expression.

These cells are sensitive to endotoxin.

LPS levels of 1 to 10 ng/ml inhibit replication by 50%.

LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/ml for extended periods.

The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities.

Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; heat inactivated fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by transferring floating cells to additional flasks. Adherent cells may be harvested by scraping. Upon reseeding, about one half of the cells will re-attach. Cultures are most successful when set up at a floating cell concentration of 1 to 4 X 10 exp5 viable cells/ml.

Medium Renewal: NR8383 [AgC11x3A, NR8383.1]細胞Two to three times weekly

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

purified RNA:ATCC CRL-2192R

References: 22160: Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174

22316: Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814

22674: Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918

22848: Limper AH, Standing JE. Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269

22970: Hidalgo HA, et al. NR8383 [AgC11x3A, NR8383.1]細胞The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855

23173: Denholm EM, Rollins SM. Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254

23190: Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284

23369: Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068

23484: Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017

23566: Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382

36466: Huang S, et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243