RL-65細(xì)胞

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

器官來源： 肺

運(yùn)輸方式： 凍存運(yùn)輸

品系： Sprague-Dawley

年限： 5 days

ATCC Number： CRL-10354?

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

RL-65細(xì)胞Designations: RL-65

Depositors: Genentech, Inc.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: lung

Strain: Sprague-Dawley

Cellular Products: cytoskeletal proteins (alpha keratin, actin, desmin, vimentin, tubulin); fibronectin; laminin

Permits/Forms: RL-65細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Age: 5 days

Comments: The cells exhibit different characteristic when grown with or without retinoic acid.

In the presence of retinoic acid (50 nM), the cells resemble low non-keratinized or squamous epithelium with densely packed colonies.

In the absence of retinoic acid, the cells form a keratinized epithelium.

Long term cultures in the absence of retinoic acid form a stratified highly keratinized epithelium with large networks of epithelial filaments.

The cells are maintained in a serum free medium.

If grown in media containing serum, the properties of the cells will change.

Propagation: RL-65細(xì)胞ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 1.2 g/L sodium bicarbonate and supplemented with 0.005 mg/ml insulin, 0.01 mg/ml human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, and bovine pituitary extract (0.15 mg protein per ml).

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture to sit at room temperature (or 37C) until the cells detach (about 5 minutes).

Centrifuge, resuspend cells in fresh medium and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 22065: Mather JP, Roberts PE. RL-65細(xì)胞Method of isolating lung cell line. US Patent 5,364,785 dated Nov 15 1994

22473: Roberts PE, et al. A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype. Am. J. Physiol. 259: L415-L425, 1990. PubMed: 2260675