4E11細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： HB-9259?
細(xì)胞形態(tài)： **樣
**類型： IgG2b
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
4E11細(xì)胞Designations: 4E11
Depositors: Immunex Corporation
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 4E11細(xì)胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with DYKDDDDK.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reaction with the peptide is calcium dependent and the antibody can be used to isolate fusion proteins tagged with the DYKDDDDK peptide.
The resulting antigen - antibody complex can be dissociated by removal of Ca++ from the buffer.
Propagation: 4E11細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 4009: Hopp TP, Prickett KS. Immunoaffinity purification system. 4E11細(xì)胞US Patent 4,851,341 dated Jul 25 1989
