6E6細胞

ATCC Number： CRL-11398?

是否是腫瘤細胞： 0

物種來源： 其他

細胞形態(tài)： 上皮樣

**類型： IgG4 kappa

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

器官來源： 卵巢

數(shù)量： 大量

6E6細胞Designations: 6E6

Depositors: ICOS Corporation

Isotype: IgG4 kappa

Biosafety Level: 2 [Cells contain SV-40-CMV viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: loosely adherent

Organism: Cricetulus griseus deposited as hamster, Chinese

Morphology: epithelial

Source: Organ: ovary

Permits/Forms: 6E6細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: female

Comments: This line is Neomycin resistant.

The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC -HB-11081).

The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.

The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins.

The cell line is capable of producing about 5-10 micrograms/liter of the humanized immunoglobulin at the rate of 1 ng/cell/day.

Propagation: 6E6細胞ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; heat-inactivated fetal bovine serum, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: 6E6細胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 45105: Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998

46120: Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158

46122: Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin,reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282