SFR8-B6細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2b

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

ATCC Number： HB-152?

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

Designations: SFR8-B6

Depositors: SF Radka

Isotype: IgG2b

SFR8-B6細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA Bw6

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SFR8-B6細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.

Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

SFR8-B6細(xì)胞fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1002: Radka SF, et al. A monoclonal antibody directed against the HLA-BW6 epitope. J. Immunol. 128: 2804-2806, 1982. PubMed: 6176653

32497: White CA, et al. Constitutive transduction of peptide transporter and HLA genes restores antigen processing function and cytotoxic T cell-mediated immune recognition of human melanoma cells. Int. J. Cancer 75: 590-595, 1998. SFR8-B6細(xì)胞PubMed: 9466661