SIRC細胞

器官來源： 其他

細胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

ATCC Number： CCL-60?

運輸方式： 凍存運輸

相關**： 正常

是否是腫瘤細胞： 0

SIRC細胞物種來源： 其他

Designations: SIRC [Statens Seruminstitut Rabbit Cornea]

Depositors: J Leerhoy

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Oryctolagus cuniculus

Morphology: fibroblast

Source: Organ: cornea

Disease: normal

Permits/Forms: SIRC細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Copenhagen Denmark

Isolation date: 1957

Virus Susceptibility: Rubella virus

Cytogenetic Analysis: modal number = 66; range = 51 to 90

The modal number (66) does not permit sexing of donor cytologically. Most cells contain one long, unpaired, submetacentric chromosome and 2 to 3 small telocentric chromosomes with satellites. Polyploidy was observed in 5/200 metaphase cells.

Comments: The SIRC cell line was derived by M. Volkert of the Staatens Seruminstitut, Copenhagen, Denmark, from the cornea of a normal rabbit in 1957. Little is recorded relating to the history of this cell line for approximately the first 400 passages. In 1965, J. Leerhoy [PubMed ID: 14331182] found this line to be susceptible to rubella virus. C.A. Phillips, et al. [PubMed ID: 5918951] confirmed the above findings and demonstrated the suitability of the cell line for primary isolation of rubella virus. The early appearance of distinct cytopathic changes makes this cell line highly suitable for both the propagation and quantitation of rubella virus [PubMed ID: 6042441].

Propagation: SIRC細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: SIRC細胞Twice per week

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2021

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 22240: Leerhoy J. Cytopathic effect of rubella virus in a rabbit-cornea cell line. Science 149: 633-634, 1965. PubMed: 14331182

26212: Phillips CA, et al. Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 122: 783-786, 1966. PubMed: 5918951

26213: Rhim JS, et al. Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 125: 1271-1274, 1967. PubMed: 6042441

53245: Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741