C171 [Clone 171]細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態(tài)： **樣
**類型： IgG1; kappa light chain
ATCC Number： HB-9515?
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: C171 [Clone 171]
Depositors: The United States of America
Isotype: IgG1; kappa light chain
C171 [Clone 171]細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. C171 [Clone 171]細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with Kunitz soybean trypsin inhibitor (KTI).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts equivalently with all 3 isoforms of KTI and binds equally well to KTI - trypsin complexes.
It does not discriminate between native and denatured KTI.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: C171 [Clone 171]細胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3945: Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990
21838: Brandon DL, et alAntigenicity of soybean protease inhibitorsIn: Brandon DL, et alProtease inhibitors as cancer chemopreventive agentsNew YorkPlenum Presspp. 107-129, 1993
22504: . . J. Food Sci. 53: 97-101, 1988.
23495: . . J. Agric. Food Chem. 36: 1336-1341, 1988.
23516: . . Crop Sci. 32: 1502-1505, 1992.
26311: . . J. Agric. C171 [Clone 171]細胞Food Chem. 39: 327-335, 1991.
