AML-193細(xì)胞

生長狀態(tài)： 懸浮生長

器官來源： 外周血

年限： 13 years

細(xì)胞形態(tài)： **樣

數(shù)量： 大量

細(xì)胞類型： 其他細(xì)胞類型

ATCC Number： CRL-9589?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 0

物種來源： 人

Designations: AML-193

AML-193細(xì)胞Depositors: Wistar Institute

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Disease: acute monocytic leukemia

Cell Type: monocyte;

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Antigen Expression: CD15, human; Homo sapiens, expressed (75 to 95% of the cells) (75 to 95% of the cells)

DNA Profile (STR): AML-193細(xì)胞Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 12

D5S818: 9,13

D7S820: 9,12

THO1: 7,9

TPOX: 8,9

vWA: 15,17

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number is 49, occurring in 50% of cells. The rate of polyploidy is 2.2%. There is one marker chromosome, der(17)t(17;17)(p13.1;q21.3), that occurred in every cell. These cells are trisomic for N3, N6, N8, and N13, and occasionally also for N2. N17 and X chromosome had a single copy, but occasionally the X had two copies. Thus the modal karyotype of this cell line is 49, X, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17. AML-193細(xì)胞The subline with 50, X, +2, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17 and 50, XX, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3, -17 also occurred, but at a very low frequency.

Age: 13 years

Gender: female

Comments: Interleukin-3 (interleukin 3, IL-3) and granulocyte/macrophage colony stimulating factor (GM-CSF) act synergistically to stimulate growth of the cells. Granulocyte colony stimulating factor (G-CSF) also supports short term and long term growth of AML-193 cells and acts synergistically with GM-CSF in inducing proliferation of the cells.This line was formerly designated ATCC HTB-188.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin and 5 ng/ml GM-CSF, 95%; fetal bovine serum, 5%.

Temperature: 37.0°C

Growth Conditions: The cells are dependent upon GM-CSF for long term growth. Conditioned medium from U-87MG (ATCC HTB-14) may be used at a concentration of 10% to replace the GM-CSF in the medium (see J. Immunol. 139:3348- 3354, 1987).

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 3 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature:AML-193細(xì)胞 liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 22617: Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132

23467: Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218