H36.12d [Pixie 12d]細胞
細胞形態(tài)： 單核細胞/巨噬細胞
ATCC Number： CRL-2447?
運輸方式： 凍存運輸
數(shù)量： 大量
生長狀態(tài)： 混合型生長
是否是腫瘤細胞： 0
物種來源： 小鼠
Designations: H36.12d [Pixie 12d]
Depositors: RT Sawyer
H36.12d [Pixie 12d]細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent and suspension
Organism: Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage) deposited as mouse
Morphology: macrophage
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: H36.12d [Pixie 12d]細胞This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. [39923]
Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing: H36.12d [Pixie 12d]細胞Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 39923: Canono EP, Campbell PA. H36.12d [Pixie 12d]細胞Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.
