PG13/LN c8細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

品系： NIH/Swiss

細(xì)胞形態(tài)： 成纖維樣

年限： embryo

ATCC Number： CRL-10685?

數(shù)量： 大量

相關(guān)**： 正常

Designations: PG13/LN c8

PG13/LN c8細(xì)胞Depositors: Fred Hutchinson Cancer Res. Cntr.

Biosafety Level: 2 [CELLS CONTAIN RETROVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Disease: normal

Strain: NIH/Swiss

Cellular Products: a Gibbon ape Leukemia Virus (GaLV) based neomycin resistance transducing vector

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. PG13/LN c8細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Reverse Transcript: positive

Age: embryo

Comments: PG13/LN c8 cells produce a retroviral vector (LN) that transduces the neo gene and can infect cells from many mammalian species (other than mice).

The line was derived from PG13 (see ATCC CRL-10686).

Although virus production has not been observed, there is a possibility that the cells will produce a virus similar to GaLV (a moderate risk oncogenic virus) and Biosafety Level 2 or higher precautions should be taken when using this cell line.

Propagation: ATCC complete growth medium: PG13/LN c8細(xì)胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, add fresh 0.25% Trypsin, 0.02% EDTA solution and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). PG13/LN c8細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

References: 1818: Miller AD, et al. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J. Virol. 65: 2220-2224, 1991. PubMed: 1850008

4473: Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980-990, 1989. PubMed: 2631796