DBS-FRhL-2細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 成纖維細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 獼猴

ATCC Number： CL-160?

相關(guān)**： 正常

年限： fetus

DBS-FRhL-2細(xì)胞器官來(lái)源： 肺

Designations: DBS-FRhL-2

Depositors: U.S. Government

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Macaca mulatta

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: DBS-FRhL-2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: April, 1969

Tumorigenic: No

Cytogenetic Analysis: Model number = 42. Range = 37 to 46.Seventy percent of the cells are diploid and the karyotype is that of a male rhesus monkey. No structural abnormalities are evident.

Age: fetus

Gender: male

Comments: This line was formerly designated CCL-160.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: DBS-FRhL-2細(xì)胞Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 3522: Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977

22396: Wallace RE, et al. DBS-FRhL-2細(xì)胞Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695