PS 60細(xì)胞
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
**類型： IgG2b; kappa light chain
運輸方式： 凍存運輸
ATCC Number： CRL-1800?
Designations: PS 60
Depositors: H Gainer
Isotype: IgG2b; kappa light chain
PS 60細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:PS 60細(xì)胞 Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for NP-OT of rat and other species (murine, human, bovine) in both radioimmunoassays and immunohistochemical staining.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
PS 60細(xì)胞heat-inactivated fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1620: Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813
2123: Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PS 60細(xì)胞PubMed: 6085284
