4E8C12細(xì)胞

Designations: 4E8C12

Depositors: Wisconsin Alumni Res. Fndn.

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

4E8C12細(xì)胞Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli 0157:H7 strain 932)

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Tumorigenic: Yes

Comments: Animals were immunized with a rough strain of 0157:H7 4E8C12細(xì)胞(E. coli HA1, deficient in the 0157 antigen).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is specific for E coli 0157:H7 and 026:H11 both of which are enterohemorrhagic.

It reacts with a protein antigen of 13000 daltons.

Propagation: 4E8C12細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50 units/ml penicillin, 0.05 mg/ml streptomycin and 0.05 mg/ml gentamicin, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 22008: Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

22063: Doyle MP, Padhye N. 4E8C12細(xì)胞Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994