Vero細胞

年限： *****

數量： 大量

細胞形態(tài)： 上皮樣

ATCC Number： CCL-81?

相關**： 正常

是否是腫瘤細胞： 0

物種來源： 綠長尾猴

器官來源： 腎臟

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

Designations: Vero

Vero細胞Depositors: W Hann, JS Rhim

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: epithelial

Source: Organ: kidney

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: March 27, 1962

Applications: detection of verotoxin

efficacy testing

malaria biology

media testing

Vero細胞mycoplasma testing

substrate

testing

transfection host

detection of virus in ground beef

Virus Resistance: Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa

Cytogenetic Analysis: This is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy.

Age: *****

Comments: The Vero cell line was initiated from the kidney of a normal ***** African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan.

The cell line was brought to the Laboratory of Tropical Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health in the 93rd passage from Chiba University by B. Simizu on June 15, 1964.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. Vero細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derivative:ATCC CRL-1587

References: 11019: British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

18385: Didier ES, et al. Vero細胞Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

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26241: Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492

26242: Liebhaber H, et al. Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 636-643, 1967. PubMed: 4961494

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26246: Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683

26247: Ozawa Y. Studies on the replication of African horse-sickness virus in two different cell line cultures. Arch. Gesamte Virusforsch. 21: 155-169, 1967. PubMed: 4232530

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