CCD-1118Sk細(xì)胞
器官來源： 皮膚
生長狀態(tài)： 貼壁生長
年限： 59 years
ATCC Number： CRL-2466?
相關(guān)**： 正常
細(xì)胞形態(tài)： 成纖維樣
細(xì)胞類型： 成纖維細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 人
數(shù)量： 大量
運輸方式： 凍存運輸
Designations: CCD-1118Sk
CCD-1118Sk細(xì)胞Depositors: A.Thompson, ATCC
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: fibroblast
Source: Organ: skin
Disease: normal
Cell Type: CCD-1118Sk細(xì)胞fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 59 years
Gender: female
Ethnicity: Caucasian
Comments: The line was established from skin taken from normal breast tissue.
Cells senesce after approximately 20 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: CCD-1118Sk細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium CCD-1118Sk細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
