BpRc1細(xì)胞

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

品系： C57L

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

器官來(lái)源： 肝

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2217?

相關(guān)**： 肝癌

Designations: BpRc1

Depositors: J Whitlock

BpRc1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Strain: C57L

Organ: liver

Disease: hepatoma

Cellular Products: cytochrome P450IA1

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: BpRc1細(xì)胞aryl hydrocarbon (Ah)

Comments: BpRc1 is a Class II variant of the Hepa-1clc7 (see ATCC CRL-2026) hepatoma cell line which was derived from the Hepa-1 cell line established by Bernhard, et al. This cell line along with the wild-type Hepa-1clc7 and the class I variant tao BpRcl (see ATCC CRL-2218) can be used to study dioxin (TCDD) induction of the CYPIA1 gene, which precedes increased levels of p450IA1 enzyme. The p450IAI enzyme catalyzes hydroxylase activity which results in the oxygenation of aromatic substrates such as the environmental carcinogen benzo[a]pyrene. Induction of transcription requires the aryl hydrocarbon hydroxylase (Ah) receptor to bind TCDD and transport of the TCDD receptor complex into the nucleus where interaction with a dioxin responsive enhancer (DRE) upstream of the CYPIA1 gene facilitates increased transcription.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: BpRc1細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 12 to 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. BpRc1細(xì)胞PubMed: 4362668