WIL2-NS細(xì)胞

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

年限： 5 years

ATCC Number： CRL-8155?

相關(guān)**： 其他**

器官來(lái)源： 脾

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

Designations: WIL2-NS

WIL2-NS細(xì)胞Depositors: TA Coons

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: spleen

Disease: hereditary spherocytosis

Cell Type: B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. WIL2-NS細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11

D16S539: 11,12

D5S818: 12,13

D7S820: 9,12

F13A01: 6,7

F13B: 10

FESFPS: 11,12

LPL: 9,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17,20

Age: 5 years

Gender: male

Ethnicity: Caucasian

Comments: WIL2-NS細(xì)胞This line does not secrete immunoglobulin.

The line was derived from the WIL-2 B cell line.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22789: Levy JA, et al. WIL2-NS細(xì)胞Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231

23199: Alspaugh MA, Tan EM. Antibodies to cellular antigens in Sjogren's syndrome. J. Clin. Invest. 55: 1067-1073, 1975. PubMed: 804494

26176: Vaughan JH, et al. Synthetic polypeptides and antibodies related to epstein-barr virus nuclear antigen. US Patent 4,654,419 dated Mar 31 1987

26177: Vaughan JH, et al. Assay for Epstein-Barr virus infection with solid phase bound synthetic polypeptides. US Patent 5,116,725 dated May 26 1992

26178: Vaughan JH, et al. Assay of anti-Epstein-Barr virus nuclear antigen antibodies with synthetic polypeptides. US Patent 5,122,448 dated Jun 16 1992

26179: Levy JA, et al. Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines. J. Natl. Cancer Inst. 46: 647-654, 1971. PubMed: 4323138