SKW 6.4細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： TIB-215?
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgM
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 人
Designations: SKW 6.4
SKW 6.4細(xì)胞Depositors: P Ralph
Isotype: IgM
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed
Cellular Products: immunoglobulin; monoclonal antibody
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DNA Profile (STR): SKW 6.4細(xì)胞Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Comments: The cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: SKW 6.4細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 exp5 cells/ml and maintain between 4 x 10 exp5 and 2 x 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1149: Saiki O, Ralph P. Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell line. Eur. J. Immunol. 13: 31-34, 1983. PubMed: 6339249
58055: SKW 6.4細(xì)胞Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913
