CCD-1079Sk細(xì)胞

數(shù)量： 大量

細(xì)胞類型： 成纖維細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 人

細(xì)胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

年限： newborn

器官來源： 皮膚

ATCC Number： CRL-2097?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 正常

CCD-1079Sk細(xì)胞Designations: CCD-1079Sk

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CCD-1079Sk細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12,13

D13S317: 11,12

D16S539: 9,11

D5S818: 11,12

D7S820: 12

THO1: 6,9.3

TPOX: 10,11

vWA: 17,18

Age: newborn

Gender: male

Comments: CCD-1079Sk細(xì)胞The line was established from skin taken from normal foreskin.

The cells senesce after approximately 56 population doublings.

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%, fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Preservation: CCD-1079Sk細(xì)胞Culture medium, 95%; DMSO, 5%