CE9H9細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG1; kappa light chain

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

ATCC Number： HB-127?

CE9H9細(xì)胞Designations: CE9H9

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against insulin

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Comments: Animals were immunized with zinc containing crystalline human insulin.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody binds to insulin from human, cow, pig and rabbit and to pro-insulin from cow and pig.

The antibody cross-reacts with insulin from sheep, guinea pig, chicken and fish.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: CE9H9細(xì)胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 981: CE9H9細(xì)胞Schroer JA, et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur. J. Immunol. 13: 693-700, 1983. PubMed: 6193965