83-12-5細胞

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

數(shù)量： 大量

ATCC Number： CRL-1971?

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgM

Designations: 83-12-5

Depositors: JA Bluestone

83-12-5細胞Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse Lyt 2.2 (CD8 alpha 2.2)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 83-12-5細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the BM10-5.6 cloned CTL (cytotoxic T lymphocyte) line.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts with Lyt 2.2.

The antibody will fix complement.

Propagation: ATCC complete growth medium: 83-12-5細胞Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 23458: Leo O, et al. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis. J. Immunol. 137: 3874-3880, 1986. 83-12-5細胞PubMed: 3097135