1116NS-3d細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgM

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-8019?

Designations: 1116NS-3d

Depositors: Wistar Institute

Isotype: IgM

1116NS-3d細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)

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Comments: 1116NS-3d細(xì)胞Animals were immunized with a human colonic adenocarcinoma (SW1116, ATCC CCL-233).

Spleen cells were fused with P3X63Ag8 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing: 1116NS-3d細(xì)胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3509: Koprowski H, et al. 1116NS-3d細(xì)胞Monoclonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen. US Patent 4,349,528 dated Sep 14 1982