B104-1-1細(xì)胞

年限： embryo

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

品系： NIH/Swiss

ATCC Number： CRL-1887?

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 胚胎

數(shù)量： 大量

B104-1-1細(xì)胞細(xì)胞形態(tài)： 成纖維樣

Designations: B104-1-1

Depositors: RA Weinberg

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Strain: B104-1-1細(xì)胞NIH/Swiss

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Age: embryo

Comments: This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.

The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185.

The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes.

The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor.

Propagation: B104-1-1細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.

References: 22917: Schechter AL, et al. B104-1-1細(xì)胞The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109