MM.1S細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

組織來源： neoplastic

是否是腫瘤細(xì)胞： 0

物種來源： 人

ATCC Number： CRL-2974?

相關(guān)**： immunoglobulin A lambda myeloma [

生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

年限： 42 years [16173231]

MM.1S細(xì)胞器官來源： 外周血

運(yùn)輸方式： 凍存運(yùn)輸

Designations: MM.1S

Depositors: S. Rosen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, suspension and lightly attached cells

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Tissue State: neoplastic

Cell Type: B lymphoblast

Disease:MM.1S細(xì)胞 immunoglobulin A lambda myeloma [16173230]

Cellular Products: lambda-light chain immunoglobulin [16173231]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1986

cloning method: soft agar

Receptors: glucocorticoid receptor, expressed

Antigen Expression: CD25+, CD38+, CD52+, CD59+ [16173231]

DNA Profile (STR): D5S818: 8, 12

D13S317: 13

D7S820: 10, 11

D16S539: 12

vWA: 15, 17

THO1: 7, 8

TPOX: 8

CSF1PO: 9,14

Amelogenin: X

Age: 42 years MM.1S細(xì)胞[16173231]

Gender: female

Ethnicity: Black

Comments: MM.1S is sensitive to dexamethasone. The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R (ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone. Together, CRL-2974 and CRL-2975 provide critical information about disease progression and the development of drug resistance. These cell lines represent valuable tools for elucidating the mechanisms of action of glucocorticoids and the development of new therapeutics.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers.

This is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.

Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended.

Medium renewal: Every 2 to 3 days

Preservation: MM.1S細(xì)胞Freeze medium: complete growth medium, 90%; DMSO, 10%

liquid nitrogen vapor phase

Doubling Time: approximately 72 hours

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

Recommended serum: ATCC 30-2020

Cell culture tested DMSO: ATCC 4-X

Derived from same cell line: ATCC CRL-2975

References: 16173230: Goldman-Leikin RE, et al. Characterization of a novel myeloma cell line, MM.1. J. Lab. Clin. Med. 113(3):335-345, 1989. PubMed: 2926241

16173231: Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914

16173308: Moalli PA, et al. A mechanism of resistance to glucocorticoids in multiple myeloma: transient expression of a truncated glucocorticoid receptor mRNA. Blood. 79:213-222, 1992. PubMed: 1728309