BDCM細胞
器官來源： 外周血
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 人
數(shù)量： 大量
運輸方式： 凍存運輸
年限： unknown
ATCC Number： CRL-2740?
相關(guān)**： 其他**
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
Designations: BDCM
Depositors: RA Steinman
BDCM細胞Biosafety Level: 2 [Cells contain EBV viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: acute myelogenous leukemia (AML)
Cell Type: lymphoblast B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: interleukin 3 (IL-3)
BDCM細胞Antigen Expression: CD20; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD40; Homo sapiens, expressed
CD80; Homo sapiens, expressed
CD83; Homo sapiens, expressed
CD86; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
CD1a; Homo sapiens
CD11c; Homo sapiens
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 10,11
D5S818: 12,13
D7S820: 9,11
THO1: 6,9.3
TPOX: 9,10
vWA: 16,17
Age: unknown
Gender: male
Ethnicity: unknown
Comments: BDCM細胞BDCM is a B cell line established from a pheresis sample from a patient with M5a leukemia. In addition to B cell markers, the cells have many characteristics of dendritic cells. Cells may be useful in modeling immune reactions. The cells are positive for Epstein-barr virus (EBV). They produce fascin, interleukin 1 alpha (IL-1 alpha), interleukin 2 (IL-2), interleukin 6 (IL-6), interleukin 16 (IL-16), transforming growth factor beta1 (TGF beta1), and tumor necrosis factor (TNF).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 3 X 10(5) and 3 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 61480: BDCM細胞Kharbanda S, et al. BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells. Br. J. Haematol. 119: 819-825, 2002. PubMed: 12437665
