B14FAF28-G3細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

年限： *****

細(xì)胞形態(tài)： 上皮樣

器官來源： 其他

ATCC Number： CCL-14?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 正常

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

B14FAF28-G3細(xì)胞Designations: B14FAF28-G3

Depositors: TC Hsu

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: peritoneum

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. B14FAF28-G3細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 1, 3; adenovirus 3

Age: *****

Gender: female

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

B14FAF28-G3細(xì)胞Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

References: 22296: . . Science 133: 1600, 1961.

26032: Hsu TC, et al. B14FAF28-G3細(xì)胞Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320