SV63細胞

ATCC Number： HB-8766?

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgG1

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

Designations: SV63

Depositors: Sloan-Kettering Institute for Cancer Research

Isotype: IgG1

SV63細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Cellular Products: immunoglobulin; monoclonal antibody; against human choriocarcinoma tumor cell antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) SV63細胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Comments: Animals were immunized with the GCC-SV(c) human choriocarcinoma cell line.

Spleen cells were fused with NS-1 myeloma cells.

The SV63 antibody reacts with an epitope present on the placental, intestinal, and germ cell forms of human alkaline phosphatase, but not on the liver/kidney/bone form of the enzyme.

The antibody precipitates a glycoprotein about 68000 daltons from extracts of choriocarcinoma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

HT

1 mM non-essential amino acids

Temperature: 37.0°C

Subculturing: SV63細胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Growth is enhanced by using a feeder layer of irradiated STO cells (ATCC 56-X).

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

MEM Non-Essential Amino Acid Solution, 100x: ATCC 30-2116

HT (Hypoxanthine-Thymidine): ATCC 71-X

Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

References: 21931: SV63細胞Rettig WJ, et al. Choriocarcinoma monoclonal antibodies and antibody panels. US Patent 4,851,332 dated Jul 25 1989