RPC5.4細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： TIB-12?
相關(guān)**： 多發(fā)性骨髓瘤
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
品系： BALB/c
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
RPC5.4細(xì)胞Designations: RPC5.4
Depositors: B Mohit
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: RPC5.4細(xì)胞immunoglobulin; monoclonal antibody
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Antigen Expression: H-2d
Cytogenetic Analysis: modal number = 60
Comments: The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. RPC5.4細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1113: Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030
1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
58048: Schwaber J. RPC5.4細(xì)胞Human lymphocyte -- mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genet. 3: 295-302, 1977. PubMed: 305122
