411-14E10細胞
ATCC Number： CRL-2663?
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態(tài)： **樣
**類型： IgG2b; kappa light chain
數量： 大量
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: 411-14E10
411-14E10細胞Depositors: National Cancer Institute
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Permits/Forms: 411-14E10細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a synthetic polypeptide with the sequence SGFNADYEASSRC corresponding to peptides 4 to 17 of the fos oncogene (Note: There is a serine deletion at position 15 of the peptide antigen).
Spleen cells were fused with Sp2/0 myeloma cells.
In immunoblot testing, a protein of approximately 55 kD is detected in mink cells transformed by FeSV (MSTF). In HeLa cell nuclei (induced by TPA), a protein of approximately 40 kD is detected.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 411-14E10細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Dulbecco's modified Eagle's medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 56128: Flanagan-Cato LM, McEwen BS. 411-14E10細胞Pattern of Fos and Jun expression in the female rat forebrain after sexual behavior. Brain Res. 673: 53-60, 1995. PubMed: 7757479
