AR42J細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

品系： Wistar

組織來源： exocrine

生長狀態(tài)： 貼壁生長

器官來源： 胰腺

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

ATCC Number： CRL-1492?

相關(guān)**： 腫瘤

Designations: AR42J

Depositors: NW Jessop

AR42J細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: pancreas

Strain: Wistar

Tissue: exocrine

Disease: tumor

Cellular Products: amylase and other exocrine enzymes [22185]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. AR42J細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: insulin, expressed

glucocorticoid, expressed

Tumorigenic: Yes

Comments: Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20% .

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The cells cells grow slowly, in clusters. They tend to pile up and appear refractile.

Subculturing: Protocol: Monolayer never becomes confluent. Subculture when patches of cells start forming "domes".

Remove and discard culture medium.

AR42J細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 3 to 4 days. May need to only add media initially, do not fluid change until cells attach well.

Preservation: Freeze medium: Complete growth medium supplemented with an additional 30% (v/v) fetal bovine serum and 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22185: Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980.

22384: Longnecker DS, et al. AR42J細(xì)胞Transplantation of azaserine-induced carcinomas of pancreas in rats. Cancer Lett. 7: 197-202, 1979. PubMed: 509403

22884: Cockell M, et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241

22978: Roux E, et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907

23083: Seva C, et al. Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J). Cancer Res. 50: 5829-5833, 1990. PubMed: 2393852

23152: Rajasekaran AK, et al. Structural reorganization of the rough endoplasmic reticulum without size expansion accounts for dexamethasone-induced secretory activity in AR42J cells. J. Cell Sci. 105: 333-345, 1993. PubMed: 7691838

23222: Longnecker DS, et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J. Natl. Cancer Inst. 58: 1769-1775, 1977. PubMed: 864754

23408: Huang Y, Hui DY. Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post- transcriptional activation by gastric hormones. J. Biol. Chem. 266: 6720-6725, 1991. PubMed: 2016288

23412: Menniti FS, et al. Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells. J. Biol. Chem. 268: 3850-3856, 1993. PubMed: 8382679

23421: Logsdon CD, et al. Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100: 1200-1208, 1985. PubMed: 2579957

23552: Zhao H, et al. Regulation of intracellular Ca2+ oscillation in AR42J cells. J. Biol. Chem. 265: 20856-20862, 1990. PubMed: 1701171

23554: Zhao H, Muallem S. Inhibition of inositol 1,4,5-trisphosphate-mediated Ca2+ release by Ca2+ in cells from peripheral tissues. J. Biol. Chem. 265: 21419-21422, 1990. PubMed: 2174872

23556: Ihara H, Nakanishi S. AR42J細(xì)胞Selective inhibition of expression of the substance P receptor mRNA in pancreatic acinar AR42J cells by glucocorticoids. J. Biol. Chem. 265: 22441-22445, 1990. PubMed: 1702421

48308: Adell T, et al. Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells. Cell Growth Differ. 11: 137-147, 2000. PubMed: 10768861

48309: Seva C, et al. Growth-promoting effects of glycine-extended progastrin. Science 265: 410-412, 1994. PubMed: 8023165

48311: Negre F, et al. Autocrine stimulation of AR4-2J rat pancreatic tumor cell growth by glycine-extended gastrin. Int. J. Cancer 66: 653-658, 1996. PubMed: 8647628

48312: Bertrand V, et al. Inhibition of gastrin-induced proliferation of AR4-2J cells by calcium channel antagonists. Int. J. Cancer 56: 427-432, 1994. PubMed: 7508895

57433: Mashima H, et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells. J. Clin. Invest. 97: 1647-1654, 1996. PubMed: 8601630

57434: Palgi J, et al. Transcription factor expression and hormone production in pancreatic AR42J cells. Mol. Cell. Endocrinol. 165: 41-49, 2000. PubMed: 10940482

90275: Mashima H, et al. Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor. Endocrinology 137: 3969-3976, 1993. PubMed: 8756573

90276: Silver K, Yao F. ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068