pgsB-618細胞

ATCC Number： CRL-2241?

數量： 大量

相關**： 其他**

細胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

器官來源： 卵巢

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 倉鼠

Designations: pgsB-618

Depositors: JD Esko

pgsB-618細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

Disease: galactosyltransferase I deficient

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NOTE: pgsB-618細胞This line is available subject to the following: 1.) The CHO cell mutant was deposited in the ATCC by Dr. Jeffrey D. Esko and is provided for research purposes only as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. The cells are provided with the understanding that they will not be used for commercial purposes. 2.) All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express permission from the University of Alabama at Birmingham. Commercial interests are the exclusive property of the University of Alabama at Birmingham. 3.) Any proposed commercial uses of these cells must first be negotiated with the Research Foundation, University of Alabama at Birmingham, Birmingham, AL 35294. Telephone: (205) 934-9911. 4.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication listed above.

Gender: female

Comments: PgsB-618 is a Chinese hamster ovary cell mutant deficient in galactosyltransferase I (UDP-D-galactose:xylose-1,4-D-galactosyl- transferase).

The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis.

PgsB-618 cells do not produce glycosaminoglycans.

Propagation: pgsB-618細胞ATCC complete growth medium: Ham's F12 medium, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Rinse the cell sheet with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the culture to sit at room temperature (or at 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23505: Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658

23546: Esko JD, et al. Inhibition of chondroitin and heparan sulfate biosynthesis in Chinese hamster ovary cell mutants defective in galactosyltransferase I. J. Biol. Chem. 262: 12189-12195, 1987. pgsB-618細胞PubMed: 2957376