EML Cell Line, Clone 1細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-11691?

數(shù)量： 大量

品系： BDF1

器官來源： 骨髓

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

EML Cell Line, Clone 1細(xì)胞Designations: EML Cell Line, Clone 1

Depositors: Fred Hutchinson Cancer Res. Cntr.

Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus deposited as mouse

Morphology:

Source: Organ: bone marrow

Strain: BDF1

Cell Type: basophil; infected with the retroviral vector LRARalpha403SN

Permits/Forms: EML Cell Line, Clone 1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: Bone marrow cells of male BDF1 mice were infected with the retroviral vector LRARalpha403SN.

RARalpha403 was inserted into the retroviral vector LXSN to generate the recombinant vector designated LRARalpha403SN.

RARalpha403 is a truncated cDNA that encodes a peptide of 403 amino acids containing the N-terminus and the DNA-binding domain.

RARalphacDNA contains a truncation of the sequences coding for the C-terminal 59 amino acids as well as a portion of the 3' untranslated region.

The infected cells were initially grown in media containing WEHI 3B conditioned medium as a source of murine IL-3), murine GM-CSF, human IL-1beta, human IL-6 and polybrene.

Cells were cloned by limiting dilution and recloned in semisolid culture medium containing 0.8% methylcellulose plus SCF.

EML Clone 1 is a stem cell factor-dependent lympho-hematopoietic progenitor cell line. EML Cell Line, Clone 1細(xì)胞They can be induced with IL-7 and bone marrow stromal cells to express characteristics of pre-pro-B lymphocytes.

EML contains progenitors capable of differentiation along B-lymphocyte, erythrocyte, neutrophil, macrophage, mast cell, and megakaryocyte lineages. They are the only known SCF-dependent cell line with both lympho and myelo-erythroid potential.

Available only as a frozen ampule.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate containing 200 ng/ml mouse stem cell factor, 80%; heat-inactivated fetal bovine serum, 20%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 3 X 10(5) viable cells/ml.

Interval: Maintain cultures at cell concentrations between 4 X 10(5) and 2 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: EML Cell Line, Clone 1細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

References: 39126: Weiler SR, et al. D3: a gene induced during myeloid cell differentiation of Linlo c-Kit+ Sca-1(+) progenitor cells. Blood 93: 527-536, 1999. PubMed: 9885214

45099: Tsai S, Collins SJ. Creating novel hematopoietic cell lines by expressing altered retinoic acid receptors. US Patent 5,830,760 dated Nov 3 1998

49678: Johnson BS, et al. Retinoid X receptor (RXR) agonist-induced activation of dominant-negative RXR-retinoic acid receptor alpha403 heterodimers is developmentally regulated during myeloid differentiation. Mol. Cell. Biol. 19: 3372-3382, 1999. PubMed: 10207061

49679: Du Y, et al. Identification of an interleukin-3-regulated aldoketo reductase gene in myeloid cells which may function in autocrine regulation of myelopoiesis. J. Biol. Chem. 275: 6724-6732, 2000. PubMed: 10702227

49683: Lawson ND, et al. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Exp. Hematol. 26: 1178-1185, 1998. PubMed: 9808058

49684: Lawson ND, et al. Isolation and characterization of the cDNA for mouse neutrophil collagenase: demonstration of shared negative regulatory pathways for neutrophil secondary granule protein gene expression. Blood 91: 2517-2524, 1998. PubMed: 9516153

50637: Lawson ND, Berliner N. Representational difference analysis of a committed myeloid progenitor cell line reveals evidence for bilineage potential. Proc. Natl. Acad. Sci. USA 95: 10129-10133, 1998. PubMed: 9707612

50638: Aoyama K, et al. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood 15: 2586-2594, 1999. PubMed: 10194438