JeKo-1細(xì)胞

年限： 78

器官來(lái)源： 外周血

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： CRL-3006?

細(xì)胞形態(tài)： **樣

運(yùn)輸方式： 凍存運(yùn)輸

組織來(lái)源： Mantle Cell Lymphoma

Designations: JeKo-1

JeKo-1細(xì)胞Depositors: Elias Campo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Characterization Data

Source: Organ: peripheral blood

Tissue: Mantle Cell Lymphoma

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Antigen Expression: CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

JeKo-1細(xì)胞DNA Profile (STR): Amelogenin:X

CSF1PO:9,12

D13S317:8,9

D16S539:12

D5S818:10,13

D7S820:10,11

THO1:7

TPOX:8

vWA: 14

Age: 78

Gender: female

Comments: The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

Propagation:JeKo-1細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: 5% CO2 in air recommended

Temperature: 37.0°C

Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

Preservation: Freeze medium: 30% RPMI; 60% FBS; 10% DMSO

Storage temperature: liquid nitrogen vapor phase

References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

16172573: Camps, J., et al. JeKo-1細(xì)胞Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063